佳节过后的我们乘着列车奔赴工作地方,一条条轨道是紧密连接两地的桥梁,使我们与亲人顺畅实现聚离。其实在许多常规实验的检测方法如ELISA、IHC、WB、FC、等,亦会用到一个类似的桥连:Biotin—Streptavidin。
这对具有高亲和力的蛋白常被偶联到抗体或蛋白,其中biotin常被偶联到抗体或配体,streptavidin偶联标记物如荧光素、HRP或AP、NanoGold等,如此通过Biotin—Streptavidin桥连来可实现间接检测靶标。
ep:ELISA(双抗夹心法):
那么,我们如何实现Biotin、Streptavidin与抗体或蛋白偶联呢?小达君郑重为您推荐Expedeon(收购Innova Bioscience)Lightning-Link®产品线中的两种桥连蛋白偶联试剂盒。它可以帮您简单快速实现偶联,无需分离直接用于后续实验,另外也省却自建偶联体系摸索条件的繁杂。
产品信息如下:
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Lightning-Link® Rapid Biotinylation Kit Type A
网址链接:
https://www.expedeon.com/products/applications/immunoprecipitaion/lightning-link-rapid-biotin/
Lightning-Link® Rapid Biotinylation Kit Type B
网址链接:
https://www.expedeon.com/products/protein-antibody-conjugation/lightning-link-antibody-labeling-kits/biotin-streptavidin-labeling-kits/lightning-link-rapid-biotin-2/
Lightning-Link®Streptavidin
网址链接:
https://www.expedeon.com/products/protein-antibody-conjugation/lightning-link-antibody-labeling-kits/biotin-streptavidin-labeling-kits/lightning-link-streptavidin/
偶联操作后怎么确认偶联上了没有?Check&Go为您分忧!厂家针对每一种偶联标记试剂盒提供相应的Check&Go产品,辅助快速检查偶联是否成功。
产品信息如下:
BiotinCheck&Go!
网址链接:
https://www.expedeon.com/products/protein-antibody-conjugation/checkgo/biotin-checkgo/
Streptavidin Check&Go!
网址链接:
https://www.expedeon.com/products/protein-antibody-conjugation/checkgo/streptavidin-checkgo/
除此之外,小达君也向您推荐streptavidin已偶联标记物产品供选择,轻松避免自行偶联的麻烦~
产品信息如下:
Streptavidin fluorescent conjugates |
Streptavidin enzyme conjugates |
Streptavidin gold nanoparticle conjugates |
Streptavidin-RPE (2012-0100/2012-1000) |
Streptavidin-alkaline phosphatase (2011-0100/2011-1000) |
20nm streptavidin-gold nanoparticle conjugate(10 OD) (251-0200/251-1000) |
Streptavidin-fluorescein (2014-0100/2014-1000) |
Streptavidin-HRP ( 2010-0100/2010-1000) |
40nm streptavidin-gold nanoparticle conjugate (10 OD) (250-0200/250-1000) |
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10nm Streptavidin gold conjugate (10OD) (252-0200/252-1000) |
小福利—Biotin Type A试用装: