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pluriSelect—让阳选后的细胞与磁珠Say goodbye!
回顾上期（Cell Strainer-细胞过滤和纯化的好帮手）pluriSelect公司不同孔径规格的细胞筛网Mini Strainer和pluriStrainer深受大家的青睐。
小达君化身大白给细胞分选加些料，助力大家的分选实验。今天介绍pluriSelect的一款分选的利器，不用千篇的内容就能掌握细胞分选的新思路， pluriSelect支招来搞定细胞阳选后的磁珠！
阳选后细胞携带的磁珠也可以去掉，这是个神马操作呢？这么好的idea，全血分选当然不在话下，并且分选后血小板或红细胞的污染非常低，得到的细胞也可以用于下游另一种细胞的阳性或阴性富集实验。可分选的样本类型也包括 PBMC, Buffy Coat, Cord Blood, Bone Marrow,Primary Cell Solution.
分选原理如下：
人的分选的指标如下（规格均为2ml装）:
|
货号 |
产品名称 |
|
19-00200-10 |
CD2 S-pluriBead® anti-hu, 2ml |
|
19-00300-10 |
CD3 S-pluriBead® anti-hu, 2ml |
|
19-00400-10 |
CD4 S-pluriBead® anti-hu, 2ml |
|
19-00500-10 |
CD5 S-pluriBead® anti-hu, 2ml |
|
19-00700-10 |
CD7 S-pluriBead® anti-hu, 2ml |
|
19-00800-10 |
CD8 S-pluriBead® anti-hu, 2ml |
|
19-00900-10 |
CD9 S-pluriBead® anti-hu, 2ml |
|
19-011a0-10 |
CD11a S-pluriBead® anti-hu, 2ml |
|
19-011b0-10 |
CD11b S-pluriBead® anti-hu, 2ml |
|
19-01400-10 |
CD14 S-pluriBead® anti-hu, 2ml |
|
19-01500-10 |
CD15 S-pluriBead® anti-hu, 2ml |
|
19-01600-10 |
CD16 S-pluriBead® anti-hu, 2ml |
|
19-01900-10 |
CD19 S-pluriBead® anti-hu, 2ml |
|
19-02100-10 |
CD21 S-pluriBead® anti-hu, 2ml |
|
19-03100-10 |
CD31 S-pluriBead® anti-hu, 2ml |
|
19-04300-10 |
CD43 S-pluriBead® anti-hu, 2ml |
|
19-04400-10 |
CD44 S-pluriBead® anti-hu, 2ml |
|
19-04500-10 |
CD45 S-pluriBead® anti-hu, 2ml |
|
19-04700-10 |
CD47 S-pluriBead® anti-hu, 2ml |
|
19-04300-10 |
CD43 S-pluriBead® anti-hu, 2ml |
|
19-04400-10 |
CD44 S-pluriBead® anti-hu, 2ml |
|
19-04400-20 |
CD44 M-pluriBead® anti-hu, 2ml |
|
19-04500-10 |
CD45 S-pluriBead® anti-hu, 2ml |
|
19-04700-10 |
CD47 S-pluriBead® anti-hu, 2ml |
|
19-05500-10 |
CD55 S-pluriBead® anti-hu, 2ml |
|
19-09500-10 |
CD95 S-pluriBead® anti-hu, 2ml |
|
19-11700-10 |
CD117 S-pluriBead® anti-hu, 2ml |
|
19-13800-10 |
CD138 S-pluriBead® anti-hu, 2ml |
|
19-19300-10 |
CD193 S-pluriBead® anti-hu, 2ml |
|
19-235a0-10 |
CD235a S-pluriBead® anti-hu, 2ml |
|
19-27100-10 |
CD271 S-pluriBead® anti-hu, 2ml |
大鼠、小鼠、猪、羊、牛、狗、马、兔子的分选指标如下（规格均为2ml装）：
|
货号 |
产品名称 |
|
22-00400-10 |
CD4 S-pluriBead® anti-rat, 2ml |
|
22-008b0-10 |
CD8b S-pluriBead® anti-rat, 2ml |
|
22-011b0-10 |
CD11b S-pluriBead® anti-rat, 2ml |
|
22-04400-10 |
CD44 S-pluriBead® anti-rat, 2ml |
|
22-04500-10 |
CD45 S-pluriBead® anti-rat, 2ml |
|
22-05500-10 |
CD55 S-pluriBead® anti-rat, 2ml |
|
22-TCRAB-10 |
Anti TCRAB S-pluriBead® anti-rat, 2ml |
|
23-011b0-10 |
CD11b S-pluriBead® anti-porcine, 2ml |
|
23-01400-10 |
CD14 S-pluriBead® anti-porcine, 2ml |
|
23-02100-10 |
CD21 S-pluriBead® anti-porcine, 2ml |
|
23-02900-10 |
CD29 S-pluriBead® anti-porcine, 2ml |
|
23-04400-10 |
CD44 S-pluriBead® anti-porcine, 2ml |
|
23-04700-10 |
CD47 S-pluriBead® anti-porcine, 2ml |
|
23-062L0-10 |
CD62L S-pluriBead® anti-porcine, 2ml |
|
23-09000-10 |
CD90 S-pluriBead® anti-porcine, 2ml |
|
23-HLARP-10 |
HLARP S-pluriBead® anti-porcine, 2ml |
|
23-RBC00-10 |
RBC S-pluriBead® anti-porcine, 2ml |
|
24-02900-10 |
CD29 S-pluriBead® anti-sheep, 2ml |
|
24-04100-10 |
CD41/61 S-pluriBead® anti-sheep, 2ml |
|
24-04400-10 |
CD44 S-pluriBead® anti-sheep, 2ml |
|
24-04500-10 |
CD45 S-pluriBead® anti-sheep, 2ml |
|
24-04700-10 |
CD47 S-pluriBead® anti-sheep, 2ml |
|
24-062L0-10 |
CD62L S-pluriBead® anti-sheep, 2ml |
|
24-27100-10 |
CD271 S-pluriBead® anti-sheep, 2ml |
|
24-HLARP-10 |
HLARP S-pluriBead® anti-sheep, 2ml |
|
25-00900-10 |
CD9 S-pluriBead® anti-bovine, 2ml |
|
25-01400-10 |
CD14 S-pluriBead® anti-bovine, 2ml |
|
25-02100-10 |
CD21 S-pluriBead® anti-bovine, 2ml |
|
25-045R0-10 |
CD45R S-pluriBead® anti-bovine, 2ml |
|
25-04700-10 |
CD47 S-pluriBead® anti-bovine, 2ml |
|
25-062L0-10 |
CD62L S-pluriBead® anti-bovine, 2ml |
|
25-11700-10 |
CD117 S-pluriBead® anti-bovine, 2ml |
|
25-HLARP-10 |
HLARP S-pluriBead® anti-bovine, 2ml |
|
27-00400-10 |
CD4 S-pluriBead® anti-canine, 2ml |
|
27-02900-10 |
CD29 S-pluriBead® anti-canine, 2ml |
|
29-00300-10 |
CD3 S-pluriBead® anti-ms, 2ml |
|
29-00400-10 |
CD4 S-pluriBead® anti-ms, 2ml |
|
29-00800-10 |
CD8 S-pluriBead® anti-ms, 2ml |
|
29-011b0-10 |
CD11b S-pluriBead® anti-ms, 2ml |
|
29-011c0-10 |
CD11c S-pluriBead® anti-ms, 2ml |
|
29-01400-10 |
CD14 S-pluriBead® anti-ms, 2ml |
|
29-01900-10 |
CD19 S-pluriBead® anti-ms, 2ml |
|
29-03100-10 |
CD31 S-pluriBead® anti-ms, 2ml |
|
29-04500-10 |
CD45 S-pluriBead® anti-ms, 2ml |
|
29-32600-10 |
CD326 S-pluriBead® anti-ms, 2ml |
|
29-F4800-10 |
F4/80 S-pluriBead® anti-ms, 2ml |
|
29-Ly6G0-10 |
Ly6G S-pluriBead® anti-ms, 2ml |
|
31-GTaHS-10 |
universal S-pluriBead® anti-hamster, 2ml |
|
31-GTaMS-10 |
universal S-pluriBead® anti-ms, 2ml |
|
31-GTaRB-10 |
universal S-pluriBead® anti-rabbit, 2ml |
|
31-GTaRT-10 |
universal S-pluriBead® anti-rat, 2ml |
|
31-RBaGT-10 |
universal S-pluriBead® anti-goat, 2ml |
|
31-RBaHU-10 |
universal S-pluriBead® anti-hu, 2ml |
|
32-011a0-10 |
CD11a S-pluriBead® anti-horse, 2ml |
|
32-04100-10 |
CD41/61 S-pluriBead® anti-horse, 2ml |
|
33-01400-10 |
CD14 S-pluriBead® anti-rabbit, 2ml |
|
33-08100-10 |
CD81 S-pluriBead® anti-rabbit, 2ml |
不同来源的样本的分选体系：
|
样本类型 |
分选体系 |
|
Whole Blood |
40 µl S-pluriBead® suspension per 1 ml |
|
Other samples |
40 µl S-pluriBead® suspension per 1x106 targets |
|
Alternatively |
40 µl S-pluriBead® suspension per 1x106 total cells |
完成分选实验需要搭配如下的辅助试剂：
|
货号 |
产品名称 |
|
50-01010-80 |
pluriPlix（用于样本混匀孵育） |
|
70-50010-11 |
S-pluriBead® Mini Reagent Kit（Kit里的各成分可单独订购） |
|
70-50010-12 |
S-pluriBead® Maxi Reagent Kit（Kit里的各成分可单独订购） |
有木有发现自己的实验中需要分选的指标呢？是不是对该分选方案充满好奇呢，心动不如行动，那就联系我们下单吧！
下一期我们会推出更精彩的内容，敬请期待！
*具体实验步骤请点击：”步骤“
